iodixanol density-gradient centrifugation optiprep kit lysiso1 (Millipore)

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Millipore iodixanol density-gradient centrifugation optiprep kit lysiso1
Iodixanol Density Gradient Centrifugation Optiprep Kit Lysiso1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iodixanol+(optiprep/lysosome+isolation+kit/pm40548932-346-10-13
Average 90 stars, based on 1 article reviews

iodixanol density-gradient centrifugation optiprep kit lysiso1 - by Bioz Stars, 2026-09

90/100 stars

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BAC Assay:

Article Title: Altered trafficking and stability of polycystins underlie polycystic kidney disease
Article Snippet: .. Lung tissues from Pkd1 F/H -BAC and Pkd1 L3040H -BAC transgenic mice were fractionated on linear iodixanol OptiPrep (Sigma-Aldrich) gradients as described previously ( 17 ), with minor modifications. .. Briefly, lung tissue homogenates were prepared using the OptiPrep buffer (Axis-Shield PoC AS) and loaded onto a freshly made 5%–25% gradient.

Transgenic Assay:

other:

Article Title: A versatile and efficient method to isolate nuclei from low-input cryopreserved tissues for single-nuclei transcriptomics.
Article Snippet: Pellets were resuspended in 1 mL of nuclei washing buffer and then 1 mL of a 50% (wt/vol) solution of iodixanol (Optiprep Millipore Sigma, Burlington, USA) was added.

Article Title: Iron-restricted Mycobacterium tuberculosis exports pathogenicity factors packed in extracellular vesicles.
Article Snippet: MEVs were adjusted to 45% w/v iodixanol (OptiPrep, SigmaAldrich) by adding OptiPrep in PBS buffer to a total of 1 ml, then loaded into the bottom of a 12.5 mL ultracentrifuge tube (Beckman) and layered with decreasing percentages of OptiPrep/PBS (1 ml of a 40% concentration, 1 ml of 35%, 1 ml of 30%, 1 ml of 25%, 1 ml of 20%, and 1 ml of PBS).

Article Title: Expression and Subcellular Localization of the Kaposi's Sarcoma-Associated Herpesvirus K15P Protein during Latency and Lytic Reactivation in Primary Effusion Lymphoma Cells
Article Snippet: The PNS was applied to the top of a preformed linear density gradient prepared from successive layers of 30%, 25%, 20%, 17.5%, 15%, 12.5%, 10%, 7.5%, 5%, and 2.5% iodixanol (OptiPrep; MilliporeSigma).

Article Title: Expression and Subcellular Localization of the Kaposi's Sarcoma-Associated Herpesvirus K15P Protein during Latency and Lytic Reactivation in Primary Effusion Lymphoma Cells
Article Snippet: The PNS was applied to the top of a 194 preformed linear density gradient prepared from successive layers of 30%, 25%, 20%, 195 17.5%, 15%, 12.5%, 10%, 7.5%, 5% and 2.5% iodixanol (OptiPrep, MilliporeSigma).

Clinical Proteomics:

Article Title: Human Polyomavirus 7–Associated Pruritic Rash and Viremia in Transplant Recipients
Article Snippet: .. A total of 250 μL of plasma was layered onto an iodixanol Optiprep (Sigma) gradient and centrifuged at 234 000 × g to purify encapsidated virions, as previously described [ 13 ]. .. A portion of all fractions collected was treated with Benzonase Nuclease (Sigma) to digest nonencapsidated nucleic acids.

Article Title: Human Polyomavirus 7–Associated Pruritic Rash and Viremia in Transplant Recipients
Article Snippet: .. Optiprep A total of 250 μL of plasma was layered onto an iodixanol Optiprep (Sigma) gradient and centrifuged at 234 000 × g to purify encapsidated virions, as previously described [ 13 ]. .. A portion of all fractions collected was treated with Benzonase Nuclease (Sigma) to digest nonencapsidated nucleic acids.

Sequencing:

Article Title: Microanatomy of the Human Atherosclerotic Plaque by Single-Cell Transcriptomics
Article Snippet: Antibodies Target antigen Vendor or Source Catalog # Working concentration Lot # (preferred but not required) Persistent ID / URL CD3 DAKO 1:100 N/A www.agilent.com/dako-products CD68 Novocastra NCL-CD68KP1 1:3200 N/A www.shop.leicabiosystems.com/us/ihcish/ihc-primary-antibodies/ CD34 Ventana Medical Systems 790-2927 Manufacturers instructions N/A www.diagnostics.roche.com α-SMA Sigma A2547 1:20000 N/A www.sigmaaldrich.com Calcein AM Invitrogen C3099 1:20 N/A www.thermofisher.com/invitrogen Hoechst 33342 Invitrogen H3570 1:100 www.thermofisher.com/invitrogen Fixable viability dye – eFluor 450 eBioscience 65-0865- 18 1:1000 N/A www.thermofisher.com/ebioscience .. PECy7 eBioscience 25-9459- 42 1:20 N/A www.thermofisher.com/ebioscience CD3 - BV421 Biolegend 317343 1:20 N/A www.biolegend.com CD4 – PETR Life technologies MHC50417 1:20 N/A www.thermofisher.com CD28 - BV650 Biolegend 302946 1:20 N/A www.biolegend.com Granzyme B – PE eBioscience 12-8899- 41 1:20 N/A www.thermofisher.com/ebioscience TruStain FcX Biolegend 422302 1:250 N/A www.biolegend.com Data & Code Availability Description Source / Repository Persistent ID / URL Sequencing data Upon request Other Description Source / Repository Persistent ID / URL RPMI 1640 Gibco www.thermofisher.com Collagenase IV ThermoFisher Scientific www.thermofisher.com DNAse I Sigma www.sigmaaldrich.com Human Albumin Fraction V MP Biomedicals www.mpbio.com Flavopiridol (Alvocidib) Selleckchem www.selleckchem.com Phorbol 12-myristate 13-acetate Sigma www.sigmaaldrich.com Ionomycin Sigma www.sigmaaldrich.com Multi-Tissue Dissociation kit Miltenyi Biotec www.miltenyibiotec.com Dead cell removal kit Miltenyi Biotec www.miltenyibiotec.com Iodixanol OptiPrep, Sigma www.sigmaaldrich.com DOI [to be added] ..

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